Fig. 7

FOXO1 regulates FTH1 expression in cardiomyocytes. a Cardiomyocytes were infected with adenovirus harboring FOXO1 or NC, and then the relative expression of genes involved in ferroptosis was determined by qPCR. b The expression levels of FTH1 mRNA and FTH1 protein were analyzed by qPCR and immunoblot in cardiomyocytes infected with adenovirus harboring FOXO1 or NC. c The expression of FTH1 mRNA and FTH1 protein was analyzed by qPCR and immunoblot in cardiomyocytes transfected with si-FOXO1 or si-NC. d Sequence motifs of FOXO1 binding sites within the promoter region of FTH1. e ChIP-qPCR assay was performed using antibodies against FOXO1 or IgG in cardiomyocytes infected with adenovirus harboring FOXO1 (Ad-FOXO1) or its negative control (Ad-Con). f The mRNA and protein levels of FHT1 were determined by qPCR and immunoblot in cardiomyocytes transfected with si-FEACR or si-NC. g Cardiomyocytes were infected with the adenovirus expressing FEACR and NC, co-transfected simultaneously with si-FOXO1 or si-NC. The expression levels of FTH1 mRNA and FTH1 protein were determined. h Cardiomyocytes were infected with adenovirus carrying FEACR along with si-FOXO1 or si-NC and then exposed to H/R. The FTH1 mRNA levels were detected by qPCR. a n = 5 biological replicates; b, c n = 4 biological replicates; e–h n = 5 biological replicates. Data were mean ± SD, and the P-value was calculated by One-way ANOVA. The experiment technically repeats three times