Fig. 1

HDAC2 negatively regulated ET-1-induced CTGF production in WI-38 cells. A Cells were transfected with either 0.5 or 1 μg of HDAC2-HA plasmid or pcDNA for 24 h and were then treated with ET-1 for 2 h. The CTGF protein level was analyzed by Western blotting. Antibody specific for α-tubulin was applied to determine loading control. HA-tag was used to confirm the overexpression of HDAC2. Bars indicate the mean ± standard error of the mean (SEM; n = 4). *p < 0.05 versus the group with ET-1 stimulation. B Cells were transfected with HDAC2 siRNA for 24 h and treated with ET-1 for 2 h; subsequently, the CTGF protein level was analyzed. Bars indicate the mean ± SEM (n = 5). ^#p < 0.05 versus control, *p < 0.05 versus ET-1 treatment