Fig. 4

Sin3A and MeCP2 negatively regulated ET-1-induced CTGF production. A Cells were transfected with either 0.5 or 1 μg of Sin3A-HA plasmid or pcDNA for 24 h and then treated with ET-1 for 2 h. The protein levels of CTGF and HA-tag were evaluated through Western blotting. Bars indicate values of the mean ± SEM (n = 4). *p < 0.05 versus the ET-1-treated group. B Sin3A was knocked down through transfection with siRNA for 24 h. After 2 h of stimulation with ET-1, the CTGF protein level was evaluated using Western blotting. Bars indicate values of the mean ± SEM (n = 3). *p < 0.05 versus the ET-1-treated group. C Cells were transfected with either 0.5 or 1 μg of MeCP2-HA plasmid or pcDNA for 24 h and then treated with ET-1 for 2 h. The protein levels of CTGF and HA-tag were evaluated using Western blotting. Bars indicate values of the mean ± SEM (n = 6). *p < 0.05 versus the ET-1 stimulation group. D MeCP2 was knocked down through transfection with siRNA for 24 h. After 2 h of stimulation with ET-1, the CTGF protein level was examined using Western blotting. Bars indicate values of the mean ± SEM (n = 4). *p < 0.05 versus the ET-1-treated group