Fig. 4From: Dietary diosgenin transcriptionally down-regulated intestinal NPC1L1 expression to prevent cholesterol gallstone formation in miceDiosgenin inhibited cholesterol absorption in Caco2 cells. A mRNA expression of genes in Caco2 cells under diosgenin treatment. B Cholesterol level in Caco2 treated with diosgenin or cholesterol. C Protein level of NPC1L1 in Caco2 cell treated with diosgenin or cholesterol. The gray value ratio was plotted on the side. D Fluorescence image of cholesterol uptake in Caco2 cells under NBD-cholesterol containing medium with and without diosgenin and ezetimibe. Mean fluorescence intensity statistics are plotted on the side. Data are expressed as mean ± SEM (3 repeats per experiment). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001Back to article page