Fig. 2
Evaluation of neutralization abilities induced by the synthesized peptide sequences. Anti-sera samples were collected from the mice immune with JEV-NTE or DV/ZV-NTE. The neutralizing titers of A JEV-NTE- or B DV/ZV-NTE-immune sera were serially diluted from 1:4 to 1:512 with PBS to determine the neutralizing capacities by FRNT50. Pre-immune sera (week 0) did not show any neutralization at a 1:8 dilution (the lowest dilution tested), and thus 1:4 was set as the detection limit. Each symbol represents an individual sample, and the horizontal lines indicate the geometric mean values of the groups. One of two representative experiments was shown. Data are the means ± SD of two independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 (by Two-way ANOVA). ns not significant