Fig. 1
From: DNA replication stress and mitotic catastrophe mediate sotorasib addiction in KRASG12C-mutant cancer
Withdrawal of KRASG12C inhibitor results in the death of sotorasib-resistant KRASG12C-mutant cancer cell. A–C Growth curves of MIA-PaCa-2, NCI-H23, and LU65 cells and their sotorasib-resistant sublines MIA-SR, H23-SR, and 65-SR cultured with (+) or without (−) of 5 μM sotorasib. D, E Results of (D) annexin V/PI flow cytometry assay and (E) caspase-3 activity assay performed using the MIA-SR and H23-SR after sotorasib withdrawal. F The MIA-SR and H23-SR cells were treated with different concentrations of the caspase inhibitor QVD after sotorasib withdrawal; the cells were counted on Day 7 (MIA-SR) and 14 (H23-SR). G The MIA-SR and H23-SR cells were cultured with or without 5 μM sotorasib in the presence of different concentrations of ARS-1620; the cell numbers were counted on Day 7 (MIA-SR) and 14 (H23-SR). Data (in A–C, E–G) are presented in terms of mean ± standard deviation values of three replicates. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001