Fig. 3
From: DNA replication stress and mitotic catastrophe mediate sotorasib addiction in KRASG12C-mutant cancer
Sotorasib withdrawal induces DNA damage and replication stress. A DNA synthesis and cell cycle distribution assessed through the BrdU incorporation assay in the MIA-SR and H23-SR cells cultured with or without 5 μM sotorasib for the indicated days. B Representative images of immunofluorescence staining and quantification of γ-H2AX staining pattern performed using the MIA-SR and H23-SR cells cultured with or without 5 μM sotorasib for the indicated days. The cell nuclei were visualized using DAPI (blue). A minimum of 200 nuclei per condition were analyzed. −: < 5 foci/cell, +: > 5 foci/cell, and ++ : pan-nuclear expression. C Effects of sotorasib withdrawal on DNA damage checkpoint proteins in the MIA-SR and H23-SR cells; protein levels were measured through Western blotting. ***P < 0.001