Fig. 2

Different enrichment strategies. A Enrichment of transfection-positive cells with selectable reporter genes. B Enrichment of nuclease-active cells using surrogate reporters based on NHEJ-, SSA-, or HDR-mediated restoration of a reporter gene. C Enrichment by co-targeting. D Co-integration of a selectable reporter gene to enrich for HDR-mediated transgene integration events. The reporter gene can subsequently stay permanently in the genome, be excised, or become silent due to induced, transient reporter expression. Constructs are not to scale. GOI (gene of interest)