Table 1 Biallelic enrichment strategies
Cells | Target locus | Transgenes | Selection | Editing pre-enrichment | Editing post-enrichment | Fold increase | Ref. |
|---|---|---|---|---|---|---|---|
HEK293T | APP/PSEN1 | Introduction of restriction site/reporter genes | Antibiotic resistance | N.A |  < 82% | N.A | [167] |
HEK293T | CCR5 | Reporter genes | Antibiotic resistance | N.A | 34.1% | N.A | [8] |
HSPC | ASXL1/RUNX1/ HBB/CCR5 | Fluorescent genes | FACS | 10.4% | 94% | 9-fold | [143] |
HSPC | ASXL1 and RUNX1 | Fluorescent genes | FACS | 1.1% | 78% | 70.9-fold | [143] |
iPSC | SNCAe2 | Mutation in HA/ Fluorescent genes | FACS | 2.1% | 100% | 46.5-fold | [145] |
iPSC | SNCAe3 | Mutation in HA/ Fluorescent genes | FACS | 3.4% | 100% | 29.4-fold | [145] |
iPSC | PINK1e5 | Mutation in HA/ Fluorescent genes | FACS | 3.7% | 100% | 26.6-fold | [145] |
iPSC | SERPINA1 | Correction of E342K missense point mutation/Reporter genes | Antibiotic resistance | N.A | 40% | NA | [160] |