Fig. 2
Crosstalk between β-AR/Epac and osteopontin/CD44 signaling on Ca2+-handling proteins. A–C Representative examples and mean ± SE analysis western blot of (p-)CaMKII, (p-)RyR2 and (p-)PLN in HL-1 myocytes treated with (A) isoproterenol (B) 8-CPT-cAMP (C) osteopontin and with or without Epac1 or Epac2 siRNA. D and E Representative examples and mean ± SE analysis western blot of CD44, Epac1, (p-)CaMKII, (p-)RyR2 and (p-)PLN in HL-1 myocytes treated with (D) osteopontin or (E) 8-CPT-cAMP and with or without CD44 siRNAs or apocynin. F Representative examples and mean ± SE analysis western blot of CD44, Epac1, (p-)CaMKII, (p-)RyR2 and (p-)PLN in HL-1 myocytes treated with isoproterenol + ICI-118,551 with or without CD44 siRNAs or Rp-8-CPT-cAMPS. The relative expression of each protein was quantified to GAPDH by densitometry and normalized to the control. For all A–F, n = 4 per group. *P < 0.05 versus control by one-way ANOVA with Bonferroni’s post hoc test. ISO = isoproterenol