Fig. 7

Increased association between CD44 and Epac1 in compromised human heart.1. A Representative examples and mean ± SE analysis western blot for osteopontin, CD44, Epac1 and Epac2 in the left ventricle (LV) from patients with preserved and compromised LV contractility. The relative expression of each protein was quantified by densitometry and normalized to GAPDH. The relative expression of each protein was quantified to GAPDH by densimetry and normalized to preserved LV contractility. B Representative examples and mean ± SE analysis for co-immunoprecipitation of CD44 and Epac1/Epac2 in human heart. The input reported in B is a re-presentation of the 4 middle lanes of the western blot reported in A. The mean ± SE analysis was for precipitated protein-bound Epac1 and Epac2 to precipitated protein (CD44) ratio and cell input, both of which were quantified to GADPH and normalized to the group of preserved LV. In A and B, N = 4 for each group. *P < 0.05 between preserved LV and compromised LV by unpaired Student’s t-test. LV left ventricle