Fig. 3

Distinct single colony proteome profile of GAS post neutrophil infection. A Relative speB mRNA expression in 5448 single colonies post neutrophil infections. Each dot represents one colony (n = 4). Bars denote mean value ± SD. B Principal component analysis of the proteome of single SpeB⁻ and SpeB⁺ colonies post neutrophil infections. GAS incubated in THY media, which remained 100% SpeB⁺ were used as controls. Each dot represents proteome analysis of one GAS colony (n = 16). C, D Heat map of differentially expressed proteins on a single colony level. Displayed are Log₂ LFQ intensities (left panel), –Log₁₀ q-value (middle panel), and Log₂ fold change of the mean (right panel). Each column represents one single colony (four per condition and donor; n = 16 in total). C Protein abundance of THY vs. SpeB⁻ colonies and D SpeB⁺ vs. SpeB⁻ colonies are displayed (×, not detected)