Fig. 6

Hyper-responsiveness of human neutrophils induced by SpeB-negative GAS. A, B Time-dependent and C, D strain-dependent comparison of secretome profiles of human neutrophils exposed to 5448 or 5448AP strains. Displayed are changes in relative abundance and p-values of the original analyses in Additional file 2: Table S5 (n = 5). E Heat map highlighting some of the significant differences of protein/peptide abundance in neutrophil secretomes from (A–D). F Principal component analysis of secreted proteins post 4 h of infection of indicated strains. Each dot represents one donor (n = 5). The ellipses indicate the calculated 95% probability region for a bivariate normal distribution with an average center of groups. G Top six upregulated pathways in 5448AP infections of primary neutrophils as compared to 5448 infections. Displayed are adjusted p-values as determined by functional profiling in the Reactome database (CH, carbohydrate; AMPs, antimicrobial peptides). H Cellular compartment analyses of protein/peptides found in higher abundance in neutrophil secretomes of 5448AP infections as compared to 5448 infections. Displayed are adjusted p-values (left red axis, red bars) and number of proteins (right blue axis, blue bars). Functional profiling was performed using Reactome database (sec., secretory; gel., gelatinase; spec., specific; azur., azurophilic)