Fig. 1

KH-type splicing regulatory protein (KSRP) expression is correlated with a poor prognosis of clear cell renal cell carcinoma (ccRCC) patients and induces clonogenicity, invasion, and anoikis resistance of ccRCC cells. A Upper panel, KSRP expression was analyzed in 72 matched ccRCC tissues and their corresponding normal tissues using data from TCGA. Lower panel, Plot depicting expression levels of KSRP in ccRCC specimens (n = 69) and normal tissue samples (n = 23). The plot was made using GSE15641 from GEO. B Upper panel, IHC staining of KSRP in ccRCC specimens and normal tissue samples. Lower panel, IHC summed scores were applied to determine KSRP expression. Scale bars of black color and red color are 100 and 30 μM, respectively. C Kaplan–Meier analysis of overall survival (OS) and disease-specific survival (DSS) rates in patients with ccRCC presenting with high or low KSRP expression using data from TCGA or E-MTAB-1980. D Knockdown efficiencies of two KSRP shRNAs were determined by Western blotting in Caki-1 and 786-O cells. E–G The effects of KSRP-knockdown on the growth, invasion, and anoikis of Caki-1 and 786-O cells were respectively examined by colony formation (E), Matrigel invasion (F), and anoikis resistance (G) assays. Values are presented as the mean ± SD of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001, compared to the control group