Fig. 2

Neural precursor cell-expressed developmentally downregulated 4 like (NEDD4L) is negatively regulated by KH-type splicing regulatory protein (KSRP) and associated with a favorable prognosis in clear cell renal cell carcinoma (ccRCC). A Flowchart for integrating microRNA and cDNA microarrays regulated by KSRP and selection of NEDD4L as a potential KSRP-targeted gene. B, C Western blotting (B) and real-time qPCR (C) analyses of NEDD4L levels in ccRCC cells transfected with a KSRP shRNA, pEGFP-KSRP, or corresponding control vector. Values from (C) are presented as the mean ± SD of three independent experiments. *p < 0.05, compared to the control group. D Correlation analysis of the KIRC database (TCGA, PanCancer Atlas) using cBioPortal revealed negative correlations between mRNA levels of KSRP and NEDD4L. E NEDD4L expression was analyzed in 72 matched ccRCC tissues and their corresponding normal tissues using data from TCGA-KIRC. F NEDD4L expression levels obtained from TCGA-KIRC were compared according to the tumor size (T stages), clinical stages, and metastasis (M stages). Statistical significance was analyzed by a Wilcoxon signed-rank test. G Kaplan–Meier analysis of overall survival (OS) and disease-specific survival (DSS) rates in patients with ccRCC presenting with high or low expression of NEDD4L using data from TCGA and E-MTAB-1980