Skip to main content
Fig. 1 | Journal of Biomedical Science

Fig. 1

From: Optimized allotopic expression of mitochondrial ND6 transgene restored complex I and apoptosis deficiencies caused by LHON-linked ND6 14484T > C mutation

Fig. 1

Subcellular location of modified human ND6. A Scheme for the structure of nucleus-versions of human ND6. Six codons in mtDNA encoding ND6 were modified as universal codons. MTS of COX8 with 25  amino acids was added to initiating codon of ND6. B A carboxy terminus FLAG-tagged nucleus-versions of human ND6. C Subcellular localization of nucleus-versions human ND6 by immunofluorescence in control cybrids. FLAG-tagged ND6 (shown in green), TOM20 (nucleus-encoding mitochondrial membrane) (shown in red). Scale bars, 15 μm. D Subcellular localization of WT and MT nucleus-versions human ND6 by Western blotting with anti-FLAG, TOM20 and β-actin (cytosol). T, total cell lysate; C, cytosol; M, mitochondria

Back to article page

Journal of Biomedical Science

ISSN: 1423-0127