Fig. 2

Analysis of complex I subunits encoded by mitochondrial and nuclear genes. A Western blot analysis of mtDNA encoding proteins. Twenty μg of total cellular proteins from various cell lines were electrophoresed through a denaturing polyacrylamide gel, electroblotted, and hybridized with ND6, ND4L, ND1, ND3 and ND5 antibodies, with β-actin as a loading control. B Quantification of ND6, ND4L, ND1, ND3 and ND5 in C, CV0, CVND6, M, MV0, and MVND6 cell lines. The calculations were based on three independent determinations in each cell line. The error bars indicate standard error of the mean (SEM). P indicates significance based on Student’s t-test of the differences between M and MVND6 cell lines. C Western blot analysis of nucleus-encoding complex I subunits. Twenty μg of total cellular proteins from various cell lines were electrophoresed through a denaturing polyacrylamide gel, electroblotted, and hybridized with NDUFA10, NDUFS5, NDUFC2, NDUFA11, NDUFS2, NDUFS1 and NDUFB8 antibodies, with β-actin as a loading control