Fig. 3

Analysis of OXPHOS complexes. A In-gel activity of complexes I, II and IV. The activities of OXPHOS complexes from various cell lines after BN-PAGE were measured in the presence of specific substrates [NADH and NTB for complex I, sodium succinate, phenazine methosulfate, and NTB for complex II, DAB and cytochrome c for complex IV]. B Quantification of in-gel activities of complexes I and IV. The calculations were based on three independent determinations in each cell line. C The levels of complexes I, II and IV by BN-PAGE. Twenty micrograms of mitochondrial proteins from various cell lines were electrophoresed through a BN- gel, electroblotted and hybridized with antibodies specific for subunits of complexes I, II and IV complexes (NDUFS2 antibody for complex I, SDHB antibody for complex II, and COX5A antibody for complex IV), and with TOM20 as a loading control. D Quantification of levels of complexes I, II, and IV. The calculations were based on three independent determinations in each cell line. Graph details and symbols are explained in the legend to Fig. 2