Fig. 4
Respiration assays. A An analysis of O2 consumption in the various cell lines using different inhibitors. The OCRs were first measured on 2 × 104 cells of each cell line under basal conditions and then sequentially added to oligomycin (1.0 µM), FCCP (0.5 µM), rotenone (1.0 µM), and antimycin A (1.0 µM) at the indicated times to determine the different parameters of mitochondrial functions. B Graphs show the basal OCR, ATP-linked OCR, proton leak OCR, maximal OCR, reserve capacity, and non-mitochondrial OCR among six cell lines. The non-mitochondrial OCR was determined as the OCR after rotenone/antimycin A treatment. The basal OCR was determined as the OCR before oligomycin minus the OCR after rotenone/antimycin A treatment. The ATP-linked OCR was determined as the OCR before oligomycin minus the OCR after oligomycin. The proton leak OCR was determined as the basal OCR minus the ATP-linked OCR. The maximal OCR was determined as the OCR after FCCP minus the non-mitochondrial OCR. Reserve capacity was defined as the difference between the maximal OCR after FCCP minus the basal OCR. The average values of three independent experiments for each cell line are shown. Graph details and symbols are explained in the legend to Fig. 2