Fig. 5

Measurements of mitochondrial ATP levels and membrane potential. A ATP levels among six cell lines C, CV0, CVND6, M, MV0, and MVND6 were measured using a luciferin/luciferase assay. Cells were incubated with 10 mM glucose or 5 mM 2-DG plus 5-mM pyruvate to determine ATP generation under mitochondrial ATP synthesis. Average rates of total cellular and mitochondrial ATP level per cell line and are shown. Three independent experiments were made for each cell line. B and C Mitochondrial membrane potential analysis. B Represented flow cytometry images of the six cell lines C, CV0, CVND6, M, MV0, and MVND6 in the presence and absence of 10 μM of FCCP. C The relative ratios of JC-10 fluorescence intensities at excitation/emission of 490/530 nm and 490/590 nm in the absence and presence of FCCP (C). Three independent experiments were made for each cell line. Graph details and symbols are explained in the legend to Fig. 2