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Fig. 6 | Journal of Biomedical Science

Fig. 6

From: Optimized allotopic expression of mitochondrial ND6 transgene restored complex I and apoptosis deficiencies caused by LHON-linked ND6 14484T > C mutation

Fig. 6

Assays for ROS production. The rates of ROS generation by mitochondria in living cells from six cell lines were analyzed by a Novocyte flow cytometer (ACEA Biosciences) using the mitochondrial superoxide indicator MitoSOX-Red (5 μM). A Flow cytometry histogram showing MitoSOX-Red fluorescence of various cell lines. B The relative ratios of intensity were calculated. The average values of three independent determinations for each cell line were shown. C Western blotting analysis of anti-oxidative enzymes SOD1, SOD2 and catalase in six cell lines with β-actin as a loading control. D Quantification of SOD1, SOD2 and catalase. Three independent experiments were made for each cell line. Graph details and symbols are explained in the legend to Fig. 2

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Journal of Biomedical Science

ISSN: 1423-0127