Fig. 7

Apoptosis assays. A Annexin V/PI apoptosis assay by flow cytometry. Cells were harvested and stained with Annexin V and 1 μL of propidium iodide. The percentage of Annexin V-positive cells were then assessed. B Relative Annexin V-positive cells from various cell lines. Three independent determinations were done in each cell line. C TUNEL assays of the six cell lines C, CV0, CVND6, M, MV0, and MVND6. Arrows indicate death cells. D Immunofluorescence analysis. The distributions of cytochrome c from the six cell lines were visualized by immunofluorescent labeling with cytochrome c antibody conjugated to Alex Fluor 488 (green) and Mitotracker (red) analyzed by confocal microscopy. DAPI stained nuclei were identified by their blue fluorescence