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Fig. 9 | Journal of Biomedical Science

Fig. 9

From: Optimized allotopic expression of mitochondrial ND6 transgene restored complex I and apoptosis deficiencies caused by LHON-linked ND6 14484T > C mutation

Fig. 9

Analysis of mitophagy. A and B Immunofluorescence analysis. The distributions of LAMP1 (A) and Parkin (B) from various cell lines were visualized by immunofluorescent staining with mitochondrial dye MitoTracker (red) and labeling with LAMP1 or Parkin. Scale bars: 20 µm. C Western blot analysis. Twenty micrograms of total cellular proteins from various cell lines were electrophoresed through a denaturing polyacrylamide gel, electroblotted, and hybridized with LC3, P62, PINK1, Parkin antibodies, with β-actin as a loading control, respectively. D and E Quantification of LC3 II/(I + II), P62, PINK1 and Parkin. Three independent experiments were done for each cell line. Graph details and symbols are explained in the legend to Fig. 2

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Journal of Biomedical Science

ISSN: 1423-0127