Table 2 An overview analysis method in ctHPVDNA studies
From: Circulating tumour DNA alterations: emerging biomarker in head and neck squamous cell carcinoma
Study | Detection regions | Analysis method | Control |
|---|---|---|---|
Cao et al. [17] | HPV L1, HPV 16/18, E6, E7 | TaqMan PCR | β-globin |
Dahlstrom et al. [27] | HPV16 E6, E7 | Real-time PCR | β-actin |
Hanna et al. [54] | HPV16,18,31,33,45 E7 | Droplet digital PCR | pUC57 plasmid |
Hanna et al. [53] | HPV16,18,31,33,45 E7 | Multiplexed droplet digital PCR | pUC57 plasmid |
Damerla et al. [28] | HPV16, HPV33 | Droplet digital PCR | EIF2C1 |
Chera et al. [22] | HPV16,18,31,33,45 E7 | Droplet digital PCR | ESR1 |
Chera et al. [23] | HPV16 E6, 37; HPV18,31,33,35,37 E7 | Digital PCR | ESR1 |
Reder et al. [121] | HPV16 E6, E7 | Real-time quantitative PCR | β-globin |
Veyer et al. [158] | HPV16 E6 | Droplet digital PCR | Albumin gene |
Haring et al. [55] | HPV16 E6 | TaqMan probe-based ddPCR | UM-SCC-104, UM-SCC-105 |
Retting et al. [123] | HPV16, 18, 31, 33, 35 | Droplet digital PCR | ESR1 |