Fig. 2

Intranasal L. johnsonii administration improved alveolarization and increased VEGF expression and lung angiogenesis on postnatal day 7. A Representative lung histology and MLI on postnatal day 7. B Representative immunohistochemical staining for vWF (black arrow) and representative Western blots and semiquantitative analysis of VEGF and vascular density. Treatment with L. johnsonii significantly diminished the hyperoxia-induced increase in the MLI and significantly augmented the hyperoxia-induced decrease in VEGF protein levels and vascular density. n = 7–11. *p < 0.05, ***p < 0.001, two-way ANOVA followed by Bonferroni post hoc test