Fig. 3

Osmotin regulates MPTP/α-synuclein-induced apoptosis in vivo and in vitro. a Immunoblot results of Bcl-2, Bcl-xL, Bax, and cytochrome c levels in the SNpc of MPTP-induced mice (n = 6, biologically independent animals). b Immunoblot results of Bcl-2, Bax, PARP-1, cytochrome c, and NeuN in the striatum and SNpc of NSE-hαSyn Tg mice (n = 6, biologically independent animals). c, d Representative photomicrograph of coronal mesencephalon and striatal sections from MPTP-induced mice containing Nissl-positive neurons (n = 6, biologically independent animals). Magnification: 200×, 100×, 50×. Scale bar represents 80 μm, 40 μm, 20 μm. e Representative photomicrograph of coronal mesencephalon sections from NSE-hαSyn Tg mice containing Nissl-positive neurons (n = 6, biologically independent animals). Magnification: 200X, 100X, 50X. Scale bar represents 80 μm, 40 μm, 20 μm. f Flow cytometric analysis of Annexin V-PE apoptosis detection assays in α-synuclein (A53T)-transfected SH-SY5Y cells. The data are presented as the mean ± SD and are representative of three independent experiments performed in triplicate. Significance was determined by using one-way ANOVA with Bonferroni correction; ^#Comparison between control and MPTP/NSE-hαSyn Tg mice, *Comparison between MPTP/NSE-hαSyn Tg mice and osmotin-administered mice. ^#/*p < 0.05, ^##/**p < 0.01, and ^###/***p < 0.001