Fig. 3

In-vitro 3D hyaluronic acid (HA) and platelet-rich plasma (hPRP) promote cell activity of rat TMJ chondrocytes. For the TMJ-OA model, neocartilage of TMJ chondrocytes/collagen constructs were cultured for 4 weeks in basal control, I + T, and I + T/hPRP/HA -conditioned medium. Constructs were then histologically examined using (A) macromorphology, hematoxylin and eosin (H&E) staining, IHC staining for Col-II, and Alcian blue staining. (B) Percentage areal deposition of s-GAG by quantification of TB-stained area, and (C) percentages of Col-II deposition in TMJ condylar cartilage. (D) Schematic of the anterior, central, and posterior regions of the rat TMJ condylar head. Results (E) posterior, (F) anterior, and (G) central condylar height. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with I + T and control group using paired t test. The results are shown as mean ± SD for three replicate