Fig. 3

Imbalanced expression of antioxidant enzymes and accumulation of intracellular H₂O₂ in MERRF-iNSCs harboring the m.8344A > G mutation. A The protein expression levels of antioxidant enzymes in normal and MERRF-iPSCs. B Quantification of the proteins in Western blots was normalized with β-actin. All the data displayed as a fold of M1^Low iPSCs. C The protein expression levels of antioxidant enzymes in normal and MERRF-iNSCs. D Quantification of the antioxidant enzyme proteins in iNSCs was normalized with β-actin. All the data are expressed as the fold change of those of N iNSCs. E Intracellular levels of H₂O₂ in MERRF-iPSCs and MERRF-iNSCs were analyzed by staining cells with DCFH-dA and were quantified in comparison with those of the control (M1^Low iPSCs and M1^Low iNSCs), respectively. The fluorescence intensity is expressed as the percentage change of M1^Low. Data are presented as mean ± SEM, n = 3. *p < 0.05; **p < 0.01; ***p < 0.001