Fig. 2

CD73 effects on migration and invasion. A-B Representative staining of migrated (A) and invaded (B) FaDu and Kyse30 cells in a Boyden chamber assay with the indicated treatments. EGF: 50 ng/mL; Cet.: cetuximab 10 µg/mL; IgG: immunoglobulin control; 22E6: antagonizing anti-CD73 monoclonal antibody (5 µg/mL) (n = 3 independent experiments; all treatments were performed for 24 h). C-D Quantitative analyses of tumor cell migration (C) and invasion (D). Shown are mean with SD of n = 3 independent experiments including singular data points and fold induction values. Ns not significant; ** p < 0.01; *** p < 0.001; **** p < 0.0001