Fig. 6

Autophagy formation in LPS-stimulated MLE-12 cells regulated by SNHG16. a LC3 and b Beclin-1 levels in LPS-stimulated MLE-12 cells. Left, immunoblot assay. Right, qRT-PCR analysis. MLE-12 cells treated with 1 µM rapamycin (Rapa) as a PC. SNHG16, miR-146a and TRAF6 levels in MLE-12 cells stimulated with c different LPS concentrations for 4 h and with d 50 µg/mL LPS for different times. e SNHG16 levels (left) in SNHG16-overexpressed MLE-12 transfectants. LC3 (middle) and Beclin-1 (right) levels in SNHG16-overexpressed transfectants stimulated with 50 µg/mL LPS for 4 h. f SNHG16 levels (left) in SNHG16-silenced MLE-12 transfectants. LC3 (middle) and Beclin-1 (right) levels in SNHG16-silenced transfectants stimulated with 50 µg/mL LPS for 4 h. Values mean ± SD. Results in a–d were representative of 3 independent experiments, and in e and f were representative of 2 independent experiments with similar findings. *p < 0.05, **p < 0.01, ***p < 0.001