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Fig. 7 | Journal of Biomedical Science

Fig. 7

From: Long noncoding RNA SNHG16 regulates TLR4-mediated autophagy and NETosis formation in alveolar hemorrhage associated with systemic lupus erythematosus

Fig. 7

NETs formation in LPS-stimulated dHL-60 cells regulated by SNHG16. a LPS-stimulated dHL-60 cells to detect DNAs morphology and measure CitH3/PAD4 levels. Left, representative photographs from mock and LPS stimulation. Scale bar = 60 µm, magnification ×200. Middle left, quantification of diffused/spread NETs morphology percentage. Middle right, CitH3 levels. Right, PAD4 levels. b SNHG16, TRAF6 and miR-146a levels in dHL-60 cells stimulated with 500 ng/mL LPS for different times. c Left, SNHG16 levels in SNHG16-overexpressed HL-60 transfectants. Middle left diffused/spread NETs morphology quantification. Middle right, CitH3 levels. Right, PAD4 levels in SNHG16-overexpressed dHL-60 transfectants stimulated with 50 µg/mL LPS for 4 h. d Left, SNHG16 levels in SNHG16-silenced HL-60 transfectants Middle left, diffused/spread NETs morphology quantification. Middle right, CitH3 levels. Right, PAD4 levels in SNHG16-silenced dHL-60 transfectants stimulated with 500 ng/mL LPS for 4 h. Values are mean ± SD. Results in a and b were representative of 3 independent experiments, and in c and d were representative of 2 independent experiments with similar findings. *p < 0.05, **p < 0.01, ***p < 0.001

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Journal of Biomedical Science

ISSN: 1423-0127