Fig. 8

Up-regulated SNHG16, TLR4 and TRAF6 expression with increased apoptosis, autophagy and NETs formation in the mouse AH lungs. a Left, representative gross and histopathological photographs in the mouse lungs with no and complete hemorrhage. Right, hemorrhagic frequencies in saline- and pristane-injected mice on day 14. Scale bar = 100 µm, magnification ×100. b RBC numbers, Hb levels and Hct on day 14 in saline- and pristane-injected mice. c Anti-RNP titers on day 14 in serum samples from saline- and pristane-injected mice. d Pulmonary (top) and splenic (low) SNHG16, TLR4, TRAF6 and miR-146a levels on day 0, 4, 9 and 14 from saline- and pristane-injected mice. e IL-6, IL-8, IFN-α and MX-1 pulmonary levels on day 0, 4, 9 and 14 from saline- and pristane-injected mice. f Representative TUNEL IF staining (green) with cell nuclei counterstained with DAPI (blue). Scale bar = 25 µm, magnification ×400. g Representative CitH3 IF staining (green) with cell nuclei counterstained with Hoechst 33258 (blue). Scale bar = 12.5 µm, magnification ×800. h Representative LC3 IF staining (green) with cell nuclei counterstained with Hoechst 33258 (blue). Scale bar = 10 µm, magnification ×1000. Arrows pointing cells with positive cytoplasmic LC3 staining. i Quantification of cell numbers with positive TUNEL, colocalized CitH3/DNAs, and cytoplasmic LC3 in lung tissues. j Immunoblot assay (left) with signal intensity quantitation analysis (right) of pulmonary LC3-II expression from saline- and pristane-injected mice. k Pulmonary levels of p53, Bax, LC3 and Beclin-1 on day 0, 4, 9 and 14 from saline- and pristane-injected mice. Values are mean ± SD. Horizontal lines are mean values. Mouse numbers per group, 10 in a, b, 8 in c, 5 in d, i, 4 in e, k, 3 in j. All results in figure were representative of 2 independent experiments with similar findings. *p < 0.05, **p < 0.01, ***p < 0.001