Fig. 1

Brain damage and reactive gliosis induced by focal cerebral ischemia. A Timeline and experimental endpoints. B Cresyl violet (Nissl) staining shows focal infarcts at 7 DPI, quantified in C, and brain atrophy at 42 DPI, quantified in D. E Representative WFA (green) and IBA1 (red) staining at 7 and 42 DPI. F IBA1 immunoreactivity in the motor cortex L5. G Representative WFA (green) and GFAP (red) staining at 7 and 42 DPI. H GFAP immunoreactivity in the motor cortex L5. E, G White squares outline 600 × 600 µm regions of interest selected for analysis. Nuclei (blue) were stained with DAPI. C, D, F, H Graphs are box plots with data as dots, means as squares, medians as lines, interquartile ranges as boxes, and whiskers showing SD. Asterisks and hashes denote significant differences with the control and stroke groups, correspondingly, as indicated by two-way ANOVA and t-tests (*^,#p < 0.05, **^,##p < 0.01, ***p < 0.001), n = 7. Scale bars, 1 mm. DPI, days post ischemia; ns, not significant