Fig. 4

PNN morphology in the motor cortex L5 post stroke. A, B Maximum intensity z-projections show the morphology of the same PNN sequentially visualized using (A) SR-SIM and (B) STED microscopy. Scale bars, 5 µm. C, D High magnification single plane images, mesh vertices, and reconstructed graphs are shown for the same region visualized using (C) SR-SIM and (D) STED microscopy. Arrowheads and dash lines indicate putative synaptic pockets and neuronal surface, correspondingly. White outlines show PNN vertices, and red outlines are interstitial particles. Scale bars, 1 µm. E, F PNN facet size was quantified as average internode distance. G, H PNN facet density was quantified as average node degree. Graphs are box plots with data as dots, means as squares, medians as lines, interquartile ranges as boxes and SD as whiskers. Asterisks and hashes denote significant differences with the control and stroke groups, correspondingly, as indicated by two-way ANOVA and t-tests (*^,#p < 0.05, **p < 0.01, ***p < 0.001), (E, G) n = 7, (F, H) n = 5. DPI, days post ischemia; SR-SIM, superresolution structured illumination microscopy; STED, stimulated emission depletion; ESC, extracellular space