Fig. 6

Surface contacts between interneurons and microglia/macrophages post stroke. A Confocal Z-projections show representative IBA1 (red), Kv3.1 (green) and WFA (white) immunolabeling in the motor cortex L5. Yellow arrowheads highlight speckles of WFA-labeled material inside IBA1-labelled cell bodies at 42 DPI. The corresponding 3D rendering of IBA1 and Kv3.1 surfaces is shown below. Scale bars, 5 µm. B Surface contact between microglia/macrophages and Kv3.1⁺/PNN⁺ cells. C Surface contact between microglia/macrophages and Kv3.1⁺ neurons with and without PNNs at 7 DPI. Graphs are box plots with data as dots, means as squares, medians as lines, interquartile ranges as boxes, and whiskers showing SD. Asterisks and hashes denote significant differences with the control and 7 DPI groups, correspondingly, as indicated by two-way ANOVA and t-tests (^#p < 0.05, ***p < 0.001, ****p < 0.00001). n = 5. DPI, days post ischemia