Fig. 6

TOX4 facilitates lung cancer cell migration and invasion. A Stable overexpression of TOX4 in H1299 and A549 was evaluated by western blotting. B The effect of TOX4 overexpression on H1299 and A549 cells migration and invasion was evaluated. Quantification of migratory and invasive cell numbers are shown (*p < 0.05, **p < 0.01, ***p < 0.001). C SLCO4A1-AS1 overexpression rescued the TOX4-evoked increase in cells migration and invasion. Quantification of migratory and invasive cell numbers are shown (*p < 0.05, **p < 0.01, ***p < 0.001). D The correlation between TOX4 expression and overall survival (log rank test, p < 0.001), and first progression (log rank test, p = 0.0026) of lung cancer patients from Kaplan Meier plotter database website. E The gross appearance of pulmonary nodules dissected from NOD/SCID mice. F Number of metastatic nodules in the lung tissue (n = 5 for each group; *p < 0.05). G Representative H&E staining images of lung tissues sections and scale = 1 mm. H Immunohistochemistry staining to assess TOX4 expression in metastatic lung node. Scale = 1 mm and 250 μm