Fig. 8
From: LncRNA SLCO4A1-AS1 suppresses lung cancer progression by sequestering the TOX4-NTSR1 signaling axis
SLCO4A1-AS1/TOX4-mediate migration and invasion through NTSR1. A H1299-TOX4 cells were transfected with NTSR1 small interfering RNAs (siNTSR1) or scramble siRNA (siCTL). The effect of siRNAs was evaluated by western blotting. B The effect of NTSR1 knockdown on H1299-TOX4 cell migration and invasion was measured. Quantification of migratory and invasive cell numbers are shown (*p < 0.05). C Stable overexpression of NTSR1 in H1299 was evaluated by western blotting. D IF staining for p-FAK (red), p-paxillin (red), rhodamine-phalloidin for F-actin (red), and nuclei (DAPI, blue) in the control and H1299-NTSR1 cells, indicated by white arrows and scale bar = 20 μm. E The correlation between NTSR1 expression and overall survival of lung adenocarcinoma patients from Kaplan Meier plotter database website (log rank test, p = 0.028). F Hypothetical model for the tumor suppressor roles of SLCO4A1-AS1 in lung cancer