Fig. 3
From: Human ACE2 protein is a molecular switch controlling the mode of SARS-CoV-2 transmission
SARS-CoV-2 is transmitted among cells through intercellular extensions. a–c The super-resolution immunofluorescence images of cell-to-cell transmission assay at 24 h after coculture. S proteins (a) and N proteins (b) were stained with antibodies. vRNA (c) was detected using a vRNA probe. D: virus donor cells (SARS-CoV-2-infected hACE2-A549 cells); R: hACE2-RFP-A549 cells; arrow: vRNA in recipient cells (c); scale bar, 10 μm. d Cells were treated with cytoskeleton inhibitors during cell-to-cell transmission assay. The infection of R cells was analyzed using IFA with anti-NSP3 antibody and quantified with a high-content image analysis system. Solvent control groups were defined as 100%; the data indicated means with SD (n = 3) of each group; *p < 0.05, **p < 0.01, ***p < 0.001, determined using two-tailed unpaired Student’s t test