Fig. 10

Schematic representation of the proposed trafficking route and mechanisms of action of Rab34 in adipocytes. Rab34 is located at the Golgi apparatus in preadipocytes (1) and transferred to the LD surface upon LD biogenesis. Specifically, Rab34 is transported from the Golgi to ER-related compartments via COPI-mediated retrograde transport pathway (2) and targeted to the LD surface via ERGIC (3a) and/or ERES (3b). Once at the LDs, Rab34 could bind FABP5 (4) and recruit UBA1 (5), which would promote ubiquitination and proteasomal degradation of FABP5 (6). FABP5 clearance from LDs would prevent full activation of phosphorylated HSL (p-HSL) thus reducing lipolysis, which would ultimately result in increased lipid accumulation in LDs. The figure was partly generated using Servier Medical Art, provided by Servier, licensed under a Creative Commons Attribution 3.0 unported license