Fig. 7

TEM1 regulates the stabilization of TGF-β receptors. A The protein levels of TGFBR1 and TGFBR2 expression on NHDFs transfected with TEM1 siRNA in response to TGF-β (10 ng/ml) for 0, 5, 15, 30, 60, and 180 min are determined using Western blotting. B The mRNA levels of TGFBR1 and TGFBR2 expression from the cell lysates of transfected NHDFs are detected by real-time PCR. C The protein levels of TGFBR1, TGFBR2, cyclin D1, and GAPDH expression on transfected NHDFs after treatment with cycloheximide (CHX) (20 μg/ml) for 0, 2, 4, and 6 h are determined by Western blotting. D The protein levels of TGFBR1, TGFBR2, ubiquitin, and GAPDH expression on transfected NHDFs treated with MG132 (10 μM) for 1 h are examined by Western blotting. E The protein levels of p- SMAD2, SMAD2, p-ERK, ERK, ubiquitin, and GAPDH expression on transfected NHDFs treated with MG132 (10 μM) for 1 h and then TGF-β1 (10 ng/ml) for 15 min are assayed by Western blotting. F Lysate from NHDFs cultured with 10% FBS DMEM is co-immunoprecipitated with the TEM1 antibody and immunoblotted with the TGFBR2 antibody. G The tissue sections from normal skins (n = 4) and keloids (n = 13) are immunostained for TEM1 and TGFBR2. The nucleus is stained with DAPI. Scale bar = 100 μm. H A positive correlation between TEM1 and TGFBR2 is determined by linear regression. I This schematic illustrates the role of TEM1 in pathologic scarring. Bar graphs show mean ± SEM