Fig. 1
From: FLT3L-induced virtual memory CD8 T cells engage the immune system against tumors
FLT3L modulated Naïve CD8 T cells display memory-like phenotype. A C57BL/6 mice were administered either Alb-FLT3L (100 ug) or vehicle control on days 1, 5, and 8 for a total of 3 doses. PBMCs collected on Day 12 after initiation of treatment were assessed for virtual-memory phenotype expressions by flow cytometry. B Representative gating of CD44 and CD49d expressions on CD8 T cells. The frequencies of CD49dlow CD44high CD8 T cells for the indicated treatment groups. C–H The mean fluorescent intensity of CD49d (C), CCL5 (D), CD5 (E), Ly6C (F), NKG2D (G), and Eomes (H) in CD8 T cells for the indicated treatment groups. N = 5 mice per group. Data is represented by mean ± SEM. P values were calculated by ordinary one-way ANOVA with the Tukey–Kramer multiple comparison test, and P < 0.05 is considered statistically significant. *, **, ***, and ****Indicate p values less than 0.05, 0.01, 0.001, and 0.0001, respectively