Fig. 2
From: FLT3L-induced virtual memory CD8 T cells engage the immune system against tumors
FLT3L-induced CD44high CD8 T cells exhibit greater proliferative and effector functions. A For the assessment of T-cell activation and effector function in Alb-FLT3Linduced CD44high CD8 T cells on Day 12 after initial treatment, control and Alb-FLT3L treated mice were euthanized, and CD44 subsets were magnetically enriched from pooled lymphocytes and splenocytes. T cells were stimulated for 24 to 48 h with either CD3 and CD28 antibodies for C57BL/6 mice, or OVA257-264 (SIINFEKL) peptides for OT-1 mice. B–D The frequency of CD69+ (B), IFNγ+ (C), and Ki67+ (D) CD8 T cells in C57BL/6 mice for the indicated treatment group. E The frequencies of dividing cells in C57BL/6 mice for the indicated treatment group. F–H The frequency of CD69+ (F), IFNγ+ (G), and dividing (H) CD8 T cells in OT-1 mice for the indicated treatment group. Data is represented by mean ± SEM. p values were calculated by ordinary one-way ANOVA with the Tukey–Kramer multiple comparison test, and p < 0.05 is considered statistically significant. *, **, ***, and ****Indicate p values less than 0.05, 0.01, 0.001, and 0.0001, respectively