Fig. 4

FLT3L-induced CD44^high CD8 T cells elevate genes involved in the JAK-STAT1 signaling pathway. For bulk RNA-seq analysis, CD44^high CD8 T cells were sorted from control naïve or FLT3L-treated C57BL/6 mice. A Pearson correlation analysis between all samples. B Volcano plot of RNA-seq transcriptome data displaying the pattern of gene expression values for FLT3L-induced CD44^high CD8 T cells relative to vehicle control CD44^high CD8 T cells. Significantly differentially expressed genes (FDR-corrected p ≤ 0.05) were highlighted in red. VM-related genes, including Ccl5 and Klrl1 were highlighted in green. C, D Enrichment plots from gene set enrichment analysis (GSEA) displaying enriched biological processes, molecular functions, and cellular components associated with genes upregulated in FLT3L-induced relative to vehicle control CD44^high CD8 T cells. E–H qPCR analysis confirmed gene expressions associated with the JAK-STAT pathway. I CD44-positive immune subsets were enriched from control and Alb-FLT3L treated B6-wild type (WT) or B6-STAT1^−/− mice. After cell enrichment, cells were stimulated with CD3 and CD28 antibodies for 72 h. The frequency of Ki67+ CD8 T cells in the indicated treatment group. Data is represented by mean ± SEM. p values were calculated by ordinary one-way ANOVA with the Tukey–Kramer multiple comparison test, and p < 0.05 is considered statistically significant. *,**,***, and ****Indicate p values less than 0.05, 0.01, 0.001, and 0.0001, respectively