Fig. 5

Type I IFNs are important for the preconditioning of CD44^high CD8 T cells. For the evaluation of type I IFN signaling affecting FLT3L in the preconditioning of CD44^high CD8 T cells, antibody blockade targeting either PDCA1 or IFNAR1 was used before and between the Alb-FLT3L treatment intervals. Mice were treated with 200 ug αPDCA or 500 ug aIFNAR1. Specifically, antibody blockade was performed on Day 1, 2, 4, 5, 8, 9 and 11. Mice were treated with 50 ug Alb-FLT3L on Day 3, 7 and 10. On Day 13, peripheral blood and spleen samples were collected and processed for flow cytometry and qPCR analysis. A Representative figures of pDCs (CD11C^intMHC2^lowSiglec-H⁺). The Modal option was used in Flowjo to normalize each peak to its mode. The frequencies of pDC in the indicated treatment group. B qPCR analysis confirmed Ifna4 expression levels. C Frequencies and numbers of CD44^high CD8 T cells. D Naïve CD8 T cells were cocultured with increasing ratios of pDCs for 3 days. The frequencies of CD44^high CD8 T cells in the indicated groups. Data is represented by mean ± SEM. p values were calculated by ordinary one-way ANOVA with the Tukey–Kramer multiple comparison test, and p < 0.05 is considered statistically significant. *, **, ***, and ****Indicate p values less than 0.05, 0.01, 0.001, and 0.0001, respectively