Fig. 5
Insertion of Ar-1 into monolayers of total lipids from M. abscessus. A Variations of the surface pressure increase (ΔΠmax) as a function of the peptide concentration upon Ar-1 insertion into total lipid monolayers from M. abscessus S and R. The increase in surface pressure due to the insertion of Ar-1 into the monolayers of total lipids, spread at an initial surface pressure Πi = 30 mN/m, was continuously monitored. Data are mean values ± SD of three independent experiments. Statistical analysis was done using a non-parametric Mann–Whitney test with Prism 8.0 (Graphpad Inc): ***p < 0.001. B Binding parameters of Ar-1 into a monomolecular film of total lipids from M. abscessus S and R. Variation of surface pressure (ΔΠmax) after Ar-1 injection (1.25 µM final concentration) is represented as a function of the initial surface pressure, Πi = 10–30 mN/m