Fig. 4

Inhibition of caspase-8 restores IL-18 and IL-8 secretion in 5448AP infections. MoDCs were treated with caspase inhibitors (caspase-3: Cas3/7-Inhibitor I, Ac-DEVD-cho; caspase-8: z-IETD-fmk, pan-capase: z-VAD-fmk) and subsequently infected with 5448AP. Cytokine secretion by moDCs was measured via a multiplex assay. A The heatmap represents the log2 fold change of cytokine concentration in relation to the respective uninfected controls. Original data are displayed in B–D and Fig. S9. Original data of IL-1β (B), IL-18 (C), and IL-8 (D) concentration in supernatants of (un)infected moDCs. The data in (B–D) are displayed as box plots. Each dot represents one independent experiment with cells from one donor (n = 10). A separate uninfected control with the respective inhibitor treatment was performed for each 5448AP infection. Untreated: indicates 5448AP infection without inhibitors. Each infection was compared to its respective uninfected controls. The level of significance was determined using Mann–Whitney U-test. Exact statistical analysis is displayed in Additional file 1: Table S6