Fig. 1

Treatment of AGS cells with CAGs and CG resulted in a different degree of lipids rafts clustering. A Structures of CAGs and CG in which R(C = O) denotes an acyl chain. B and C Confocal images of lipid rafts clustering in the presence of CAGs and CG in a single (B) or multiple AGS cells (C). After AGS cells were treated with CAGs or CG (as indicated) for I h, lipid rafts (GM1-containing) were labeled with Alexa Fluor 594-conjugated cholera toxin subunit b (CT-b; red fluorescence). Confocal images were collected under a Leica SP5 X inverted confocal microscope. Scale bar: 5 μm. Please note that colon-containing numbers are designated as CAGs that contain corresponding acyl chains. D The fluorescent intensities in (C) were quantified. At least 40 GM1-positive AGS cells from each experiment were scored for quantitative analysis by using ImageJ software. Statistical analysis was performed using Student's t test, and the asterisks represent statistical significance (P < 0.05) compared with the DMSO control group. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)