Figure 4
Expression of genes unique to cancer stem cells and/or malignant cells. (A) Cell extracts from SAS-GFP and SASVO3 were analyzed for the expression of Bmi1, p14Arf, p21, pAKT, AKT and β-catenin by Western blotting. GAPDH served as a loading control, was reproducibly similar in all experiments. Results shown are from one of three similar experiments. (B) The expression of Bmi1 protein in mouse xenografts tissue from the dorsal tumor area was detected by IHC.