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Table 1 The components of solutions used in patch-clamp experiments

From: Developmental expression of a functional TASK-1 2P domain K+ channel in embryonic chick heart

Components (in mM)

S-1

S-2

S-3

P-1

P-2

NaCl

140

-

-

-

 

KCl

5.4

125

-

145

 

CaCl2

1.8

-

-

-

5

MgCl2

1.05

2

2

2

1

Dextrose

5.5

5.5

5.5

-

-

HEPES

10

10

10

10

10

EGTA

-

-

-

0.5

11

K-Aspartate

-

-

140

-

130

ATP·Mg

-

-

-

5

-

ATP·Na2

-

-

-

-

5

BaCl2

-

0.1

0.1

-

-

TEA·Cl

-

20

10

-

-

4-AP

-

0.5

0.5

-

-

  1. Note: pH of pipette solutions (P-1 and P-2) was adjusted to 7.2 with KOH. For all bath solutions pH was adjusted to 7.4 with NaOH or KOH. TEA: tetraethylammonium, 4-AP: 4-aminopyridine.
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Journal of Biomedical Science

ISSN: 1423-0127