Figure 7

Treating ASTC-a-1 cells with DHA induced alteration of mitochondrial morphology. Cells were loaded with Mito-tracker-red and then treated with 20 μg/ml DHA for analysis.a The mitochondria within the untreated control cells showed small-defined mitochondria. b The mitochondria of DHA-treated cells were obviously swollen. The right-hand panels represent enalrged views of the boxed regions within the left-hand panels, as indicated. (c, d) The spatial distribution of mitochondrial sizes indicated as the white line in a and b, respectively. e For every condition tested, the width of 100–200 mitochondria in at least 50 different cells was determined. Data analyzed with SPSS10.0 software are expressed as mean ± SD. *P < 0.05, **P < 0.01, compared with control. Scale Bar: 10 μm